Recombinant Proteins

Proteins

Recombinant Proteins

Human

BDNF

BMP2

EGF

BMP4

CD38

CD4

KLF4

TCF3 / E2A

BMP6

C-MYC

DPPA2

OCT4

SOX2

BMP7

NANOG

PDX1

POU5F1

SOX4

DNMT3B

FGF4

GATA4

SOX1

SOX10

SOX9

WNT3A

WNT8A

BMP5

BMP8B

KLF5

NODAL

OTX2

SOX11

SOX7

E. coli

HEK293T

CHO

Pichia pastoris

C-Myc/DDK

His-tag

Tag Free

N-His

C-His

Myc-DDK

C-Fc

C-Human Fc

His-Cam-tag

C-mFc

N-6His-Avi

N-GST and C-HIS

N-GST and C-His

N-T7

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Cancer stem cells

Nuclear Hormone Receptor

Induced pluripotent stem cells

P450

GPCR

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Potassium

Ion Channels: Cys-loop Receptors

Ion Channels: Glutamate Receptors

Ion Channels: ATP Receptors

Ion Channels: Transient receptor potential

Ion Channels: Sodium

ELISA

Ion Channels: Cyclic nucleotide gated

000) and Immunohistochemistry (1/200-1/1

000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

000-1/32

1/500-1/2

10 mM Tris-HCl

2 mM dithiothreitol

500)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

ELISA (1/8

FACS

Suitable for Immunoblotting (Western or Dot blot

The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography.

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

pH 7.4).
For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

Pathways in cancer

TGF-beta signaling pathway

Hedgehog signaling pathway

MAPK signaling pathway

Cytokine-cytokine receptor interaction

Hematopoietic cell lineage

Basal cell carcinoma

Endocytosis

Acute myeloid leukemia

Bladder cancer

Endometrial cancer

ErbB signaling pathway

Melanogenesis

Melanoma

Regulation of actin cytoskeleton

Huntington's disease

Neurotrophin signaling pathway

Focal adhesion

Gap junction

Glioma

Non-small cell lung cancer

Pancreatic cancer

Prostate cancer

Wnt signaling pathway

Metabolic pathways

Antigen processing and presentation

Calcium signaling pathway

Cell adhesion molecules (CAMs)

Nicotinate and nicotinamide metabolism

Primary immunodeficiency

T cell receptor signaling pathway

Cell cycle

Chronic myeloid leukemia

Colorectal cancer

Jak-STAT signaling pathway

Small cell lung cancer

Thyroid cancer

Maturity onset diabetes of the young

Type II diabetes mellitus

Cysteine and methionine metabolism

Human

E. coli

1

2

Yes

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