Native Proteins

Proteins

Native Proteins

Bovine

GFAP

10 mM Tris-HCl

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

pH 7.4).
For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

Spinal Cord

2