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Neurofilament L (68kDa) bovine protein, 0.1 mg
| Protein Source | Spinal Cord |
Native Proteins
Neurofilament L (68kDa) bovine protein, 0.25 mg
| Protein Source | Spinal Cord |
Neurofilament H (200 kD) bovine protein, 0.25 mg
| Protein Source | Spinal Cord |
GFAP bovine protein, 0.1 mg
| Protein Source | Spinal Cord |
Neurofilament M (160 kD) bovine protein, 0.25 mg
| Protein Source | Spinal Cord |
GFAP bovine protein, 0.25 mg
| Protein Source | Spinal Cord |
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Proteins
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization. (4) - 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
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pH 7.4).
For immunization purposes (2) - the solution can be further dialyzed against PBS (phosphate buffered saline (2)
