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Proteins
- Native Proteins
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Species
Target Symbol
Conjugates
Families
- Druggable Genome (7)
- 10 mM Tris-HCl (6)
- 2 mM dithiothreitol (6)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization. (6) - Secreted Protein (6)
- the solution can be further dialyzed against PBS (phosphate buffered saline (6)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (4)
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e.g. Dulbeccos PBS).
See References 2 and 3 for more details. (4) -
pH 7.4).
For Immunization purposes (4) - Protease (3)
- Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) -
Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization. (2) - Suitable for Immunoblotting (Western or Dot blot) (2)
- Transmembrane (2)
- e.g. Dulbeccos PBS). (2)
-
pH 7.4).
For immunization purposes (2) - 000-1/32 (1)
- 000. (1)
-
000.
Western Blot: 1/500-1/2 (1) -
500.
Immunohistochemistry: 1/200-1/1 (1) -
ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting. (1) -
ELISA.
Western Blot. (1) - ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays. (1)
- ELISA. (1)
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ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000. (1) -
ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8 (1) - ELISA. (1)
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ELISA.
Western Blot. (1) - ES Cell Differentiation/IPS (1)
- For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve (1)
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For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting. (1) - Specific methodologies have not been tested using this product. (1)
-
change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors (1) -
etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments) (1) - for immunoprecipitation and for Wwestern blotting. (1)
- inhibitors (1)
- optimal concentration with heparin (30 μg/ml) is about 12 μg/ml. (1)
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optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent (1) - plate cells in the morning (1)
Pathways
- Neuroactive ligand-receptor interaction (4)
- ECM-receptor interaction (3)
- Focal adhesion (3)
- Amyotrophic lateral sclerosis (ALS) (2)
- Complement and coagulation cascades (2)
- Pathogenic Escherichia coli infection (2)
- Systemic lupus erythematosus (2)
- GnRH signaling pathway (1)
- Lysosome (1)
- Pathways in cancer (1)
- Renin-angiotensin system (1)
- Small cell lung cancer (1)
