Oligo annealing buffer (10X)
Gene Expression
Cre recombinase mammalian expression vector
CF3, the forward sequencing primer to sequence target sequence cloned in CRISPR vectors.
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
Cas9 in vitro digestion buffer, 10x
CRISPR/Cas9 starter kit (HA tagging human HSP60 at C-terminus)