Antibodies

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, Purified

Applications Flow Cytometry.
Immunoprecipitation. (Ref.1)
Reactivities Chicken
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Indirect Immunofluorescence staining. (Ref.1)
Reactivities Chicken
Conjugation Biotin

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
Western blot: 1/1,000-1/10,000.
ELISA: 1/10,000-1/50,000.
Immunohistochemistry: 1/500-1/2500.
Reactivities Chicken
Conjugation HRP

Chicken IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, Purified

Applications ELISA (1/100-1/10000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, FITC

Applications Flow cytometry.
Reactivities Chicken
Conjugation FITC

Chicken IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/10,000-1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/10,000.
Reactivities Chicken
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Immunocytochemistry (1/200).
In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation FITC

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivities Chicken
Conjugation Biotin

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells. (Ref.1)
Reactivities Chicken
Conjugation PE

Chicken IgM goat polyclonal antibody, HRP

Applications ELISA (1/10,000-1/100,000).
Reactivities Chicken
Conjugation HRP