Antibodies

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy, Fluorescent based plate assays (FLISA) and Fluorescent Western Blotting.
Also Suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy : 1/1,000-1/5,000.
Reactivities Human
Conjugation FITC

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000-1/100,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.

Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgA + IgG + IgM (light chain) mouse monoclonal antibody, clone 7A9

Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 52G1

Reactivities Human
Conjugation Unconjugated

Human IgE (C epsilon 3 specific) (in vitro) mouse monoclonal antibody, clone 4F4cc

Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Human IgD mouse monoclonal antibody, clone IA6-2, PE

Applications Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities Human
Conjugation PE

Human IgD mouse monoclonal antibody, clone IA6-2, Purified

Applications Flow cytometry.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 3H10G5F2, Purified

Applications ELISA: 1/10000.
Reactivities Human
Conjugation Unconjugated

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6061, Purified

Applications ELISA: 2.5-10 µg/mL
This antibody is best used as a Capture antibody.
Reactivities Human
Conjugation Unconjugated