Antibodies

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, HRP

Applications ELISA: 1/4,000-1/8,000 (See also Ref.1).
Reactivities Human
Conjugation HRP

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/10,000-1/20,000.
Reactivities Human
Conjugation Biotin

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, PE

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Human IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Human
Conjugation FITC

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Human IgA2 mouse monoclonal antibody, clone A9604D2, Biotin

Applications ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation Biotin

Human IgA2 mouse monoclonal antibody, clone A9604D2, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, Biotin

Applications ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation Biotin

Human IgE mouse monoclonal antibody, clone 4G7, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone B537M, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated