Antibodies

Human IgM rat monoclonal antibody, clone HM-7, Aff - Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-2,500.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgG (Fc specific) (in vitro) mouse monoclonal antibody, clone 5A9cc

Reactivities Human
Conjugation Unconjugated

Human IgA (Fc specific) (in vitro) mouse monoclonal antibody, clone 3B7cc

Reactivities Human
Conjugation Unconjugated

Canine IgE mouse monoclonal antibody, clone E6-71A1, Biotin

Applications ELISA: The antibody may be used as a detection antibody in ELISA techniques in combination with SM1498P as capture antibody.
Western Blotting: Under reducing conditions, this antibody detects a band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine, Human
Conjugation Biotin

Human IgA (Secretory component) mouse monoclonal antibody, clone MC29/12, Purified

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.)
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, PE

Applications ELISA.
Immunofluorescence < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Biotin

Applications ELISA: 1/5,000-1/20,000.
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation Biotin

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Biotin

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, FITC

Applications FLISA: 1/200-1/600.
Indirect Immunofluorescent staining
of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
Reactivities Human
Conjugation FITC