Antibodies

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Azide Free

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry. 
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified

Applications

Indirect Immunostaining (In conjunction with mouse monoclonal antibodies).
Enzyme-Linked-Immunosorbent-Assay (ELISA)
Fluorescent-Linked-Immunosorbent-Assay (FLISA)
Immunoblotting. 
Flow Cytometry.

Reactivities Mouse
Conjugation Unconjugated

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
Western blot (1/5000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Immunoprecipitation (1/250).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation Unconjugated

Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/150,000.
Western Blot: 1/3,000-1/15,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Turkey IgG (H+L chain) goat polyclonal antibody, Purified

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Reactivities Turkey
Conjugation Unconjugated

Rat IgG2a goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction

Applications ELISA: 1/20000-1/100000. 
Western Blot: 1/2000-1/10000. 
Immunohistochemistry: 1/1000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Purified

Applications Fluorescent-linked-immunosorbent assay (FLISA)
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry (frozen Sections).
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Purified

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA. 1 ug/mL
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated