Antibodies

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human
Conjugation Unconjugated

Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.) 
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment 
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone B1545M, Purified

Applications
This antibody can pair with itself in ELISA, EIA.
Can be used for specific IgM antibodies detection and for total IgM level detection.
Can also be used as a capture antibody for total IgM level detection.
Reactivities Human
Conjugation Unconjugated