Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Biotin
Applications | Suitable for Western Blot, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Other applications not tested. Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the reconstitution concentration is suggested for this product. |
Reactivities | Human |
Conjugation | Biotin |