Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP
Applications | Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/2000-1/10000. Western Blot: 1/500-1/2500. Immunochemistry: 1/200-1/1000. |
Reactivities | Mouse |
Conjugation | AP |