Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free
Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
Reactivities | Sheep |
Conjugation | Unconjugated |