Antibodies

Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration
before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Frozen Sections.

Can be used to identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Erythrocytes rabbit polyclonal antibody, Azide Free

Applications ELISA, IF, WB
Reactivities Sheep

DYNC1I2 (cytoplasmic, 74kD) mouse monoclonal antibody, clone 74.1, Purified

Applications IHC, IP, WB
Reactivities Bovine, Drosophila, Mouse, Sheep, Xenopus, Zebrafish

IL-1B rabbit polyclonal antibody, Serum

Applications ELISA, FN, WB
Reactivities Sheep

CD62P (SELP) mouse monoclonal antibody, clone Psel.KO.2.12, Purified

Applications FC, IHC, IP
Reactivities Goat, Human, Rat, Sheep

IL4 mouse monoclonal antibody, clone CC313, Purified

Applications ELISA, FC
Reactivities Bovine, Goat, Sheep

IL-1B mouse monoclonal antibody, clone 1D4, Purified

Applications ELISA, FC, IHC
Reactivities Bovine, Goat, Sheep

MHC Class I (monomorphic) mouse monoclonal antibody, clone 2G5, Purified

Applications FC, IP
Reactivities Bovine, Guinea Pig, Hamster, Human, Mouse, Porcine, Sheep

IL6 mouse monoclonal antibody, clone 4B6, Purified

Applications ELISA, FC, WB
Reactivities Monkey, Sheep

Integrin alpha 6 (ITGA6) rat monoclonal antibody, clone NKI-GoH3, Purified

Applications FC, IHC, IP
Reactivities Canine, Human, Monkey, Mouse, Porcine, Sheep

IL12B mouse monoclonal antibody, clone CC326, Azide Free

Applications ELISA, FC, FN
Reactivities Bovine, Human, Sheep