Antibodies

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
General Recommended Dilutions:
Immunocytochemistry.
Immunohistochemistry (Paraffin).
Histochemical Use: 1/10-1/40.
ELISA: 1/100-1/2000. 
Western blot: 1/200-1/4000.
Reactivities Human
Conjugation Biotin

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards
ELISA: form 1/25 upwards
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Biotin

Human IgD (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Performance testing. Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Conjugation Biotin

Human IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Human
Conjugation Biotin

Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:194,000 - 1:294,000
Reactivities Human
Conjugation Biotin

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Biotin

Applications ELISA: 1/5,000-1/10,000.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Biotin

Kappa light chain, mouse anti human, clone κ -117 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Lambda light chain, mouse anti human, clone λ-1 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin

Human IgA + IgG + IgM goat polyclonal antibody, Biotin

Applications Western Blot: 1/5000-1/20000.
ELISA: 1/400000.
Immunohistochemistry on frozen and paraffin sections: 1/1000-1/5000.
Reactivities Human
Conjugation Biotin