Antibodies

Vimentin (VIM) mouse monoclonal antibody, clone RV202, FITC

Applications FC, IF, IHC
Reactivities Canine, Chicken, Goat, Human, Monkey, Mouse, Porcine, Rat, Zebrafish

OVAL rabbit polyclonal antibody, FITC, Purified

Applications ELISA, ID, IF, IHC, IP, R
Reactivities Chicken

Vimentin (VIM) mouse monoclonal antibody, clone VIM 3B4, FITC

Applications ELISA, FC, IF, IHC, WB
Reactivities Amphibian, Bovine, Canine, Chicken, Human, Monkey

Chicken IgA (Fc specific) goat polyclonal antibody, FITC

Applications Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases.
Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Immunocytochemistry: 1/10-1/40.
Immunohistochemistry: 1/10-1/40.
Reactivities Chicken
Conjugation FITC

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Assay Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-5,000.
Reactivities Chicken
Conjugation FITC

Chicken IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Chicken
Conjugation FITC

basic Cytokeratin mouse monoclonal antibody, clone AE3, FITC, Purified

Applications FC, IF
Reactivities Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

pan Cytokeratin mouse monoclonal antibody, clone AE1/AE3, FITC, Purified

Applications FC, IF
Reactivities Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat

acidic Cytokeratin mouse monoclonal antibody, clone AE1, FITC, Purified

Applications FC, IF
Reactivities Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat

acidic Cytokeratin mouse monoclonal antibody, clone AE1, FITC, Purified

Applications FC, IF
Reactivities Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat

pan Cytokeratin mouse monoclonal antibody, clone AE1/AE3, FITC, Purified

Applications FC, IF
Reactivities Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Immunocytochemistry (1/200).
In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation FITC