Antibodies

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, AP

Applications ELISA: 1:4,000 - 1:20,000.
Western blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:200 - 1:1,000.
Reactivities Mouse
Conjugation AP

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:7,000 to 1:70,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:4,000 - 1:16,000.
Western Blot: 1:500 - 1:2,000.
Reactivities Hamster
Conjugation HRP

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
ELISA: 1:20,000 - 1:100,000.
Western Blot: 1:2,000 - 1:10,000.
Reactivities Hamster
Conjugation Biotin

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:3,000 - 1:12,000.
Western Blot: 1:500 - 1:2,000.
Reactivities Hamster
Conjugation AP

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000-1:10,000.
Immunofluorescence microscopy: 1:500-1:2,500.
Reactivities Hamster
Conjugation Texas Red

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:200,000 is suggested for this product.
The following general recommendations are suggested as starting dilutions for:
Immunoblotting: 1:10,000,
Enzyme Immunohisto-chemistry on tissue sections: 1:500-1:5,000.
Flow cytometry and fluorescence Immunohisto/cytochemistry: 1:200-1:1,000.
Reactivities Goat
Conjugation Biotin

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:500 - 1:5,000.
Immunohistochemistry: 1:200 - 1:2,000.
Reactivities Goat
Conjugation AP

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Goat
Conjugation TRITC

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Reactivities Goat
Conjugation Texas Red

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:5,000 - 1:20,000.
Reactivities Goat
Conjugation Unconjugated

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation Texas Red

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation TRITC