Secondary Antibodies

Rat IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Conjugation ATTO 488

Biotin Conjugated Rabbit Anti-human IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Human IgG
Conjugation Biotin

Rabbit IgG (H+L chain) goat polyclonal antibody, Biotin, Lyophilized

Applications Western Blot: 1/5,000-1/20,000 for Western blots.
ELISA: 1/50,000-1/200,000.
Immunohistochemistry: 1/1,000-1/5,000.

Reactivities Rabbit
Conjugation Biotin

Rabbit Monoclonal Anti-Mouse Kappa Light Chain antibody, clone RM103

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse Ig kappa light chain
Conjugation Unconjugated

Bovine IgG (H+L chain) goat polyclonal antibody, Agarose

Applications Suitable for antibody purification or to remove unwanted cross-reactivity.
Reactivities Bovine
Conjugation Agarose

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Western blot: 1/1,000-1/5,000
Flow Cytometry: 1/500-1/2,500
Reactivities Mouse
Conjugation HRP

Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications FLISA: 1/100–1/400.
Flow Cytometry: ≤ 1 μg/106 cells.
Immunofluorescence: ≤ 1 μg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone 4A10, Ascites

Applications ELISA: 1/10000.
Western Blot: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Immunohistochemistry on Frozen Sections.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Mouse IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425

Rabbit IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Conjugation ATTO 425

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:500 - 1:2,500
ELISA: 1:250,000
Reactivities Human
Conjugation HRP

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:500 - 1:2,500
ELISA: 1:250,000
Reactivities Human
Conjugation HRP

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP