Secondary Antibodies

Mouse IgG2a Antibody ATTO 655 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 655

Mouse IgG2b Antibody ATTO 425 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425

Mouse IgG2b Antibody Alkaline Phosphatase Conjugated Pre-adsorbed

Applications WB: 1:500 - 1:2,000
IHC: 1:200 - 1:1,000
ELISA: 1:2,000 - 1:10,000
Reactivities Mouse
Conjugation Alkaline Phosphatase

Mouse IgG2b Antibody ATTO 488 Conjugated Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IF: >1:5,000
FC: 1:500 - 1:2,500
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 488

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Immunoblotting.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, APC

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation APC

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry: ≤ 1 µg/106 cells.
Identification and enumeration of IgG2a+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/60, depending on the method used.
Reactivities Mouse
Conjugation TRITC

Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: User Optimized
ELISA: 1:100,000
Reactivities Mouse
Conjugation HRP

Mouse IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:350,000
Reactivities Mouse
Conjugation Biotin

Mouse IgG IgA IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation HRP

Mouse IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:50,000 - 1:100,000
Reactivities Mouse
Conjugation HRP

Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:180,000
Reactivities Mouse
Conjugation HRP

Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:5,000-1:40,000
IHC: 1:500-1:5,000
ELISA: 1:10,000-1:100,000
Reactivities Mouse
Conjugation HRP

Mouse IgG (H&L) Antibody Pre-Adsorbed

Applications WB: 1:10,000 - 1:50,000
IHC: 1:5,000 - 1:25,000
ELISA: 1:100,000 - 1:500,000
Reactivities Mouse
Conjugation Unconjugated