Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, HRP
Applications | Western blot: Recommended dilution: 1 μg/ml. |
Reactivities | Human |
Conjugation | HRP |
Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, HRP
Applications | Western blot: Recommended dilution: 1 μg/ml. |
Reactivities | Human |
Conjugation | HRP |
Monkey IgG (Fc specific) goat polyclonal antibody, HRP
Applications | Dot blot. Immunoblotting. ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. This antibody can be used: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histo- and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000. |
Reactivities | Monkey |
Conjugation | HRP |
Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, PE
Applications | Flow cytometry: 0.5 µg/ml. |
Reactivities | Human |
Conjugation | PE |
Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, APC
Applications | Flow Cytometry: 2 µg/ml is recommended starting point. |
Reactivities | Human |
Conjugation | APC |
Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, FITC
Applications | Flow Cytometry: Use 50µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl. |
Reactivities | Mouse |
Conjugation | FITC |
Monkey IgA (Secretory component) goat polyclonal antibody, HRP
Applications | Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working dilutions: Histochemical and cytochemical 1/150 - 1/500. ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000. |
Reactivities | Chimpanzee, Monkey |
Conjugation | HRP |
Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP
Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
Reactivities | Porcine |
Conjugation | HRP |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
Reactivities | Monkey |
Conjugation | HRP |
FITC Conjugated Goat Anti-Mouse IgG
Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
Reactivities | Mouse IgG |
Conjugation | FITC |
Rabbit Monoclonal Anti-Human IgM antibody, clone RM121
Applications | ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL |
Reactivities | Human IgM |
Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone BE5, PE
Applications | Flow cytometry: 5 µg/ml. |
Reactivities | Human |
Conjugation | PE |
Human IgE mouse monoclonal antibody, clone BE5, FITC
Applications | Flow cytometry: 5 µg/ml. |
Reactivities | Human |
Conjugation | FITC |
Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified
Applications | Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. For use in ELISA a dilution of 1:3,000-1:12,000 is recommended. |
Reactivities | Mouse |
Conjugation | Unconjugated |
IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
Applications | Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user. |
Reactivities | Porcine |
Conjugation | Unconjugated |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE
Applications | FLISA: ? 0.1 µg/ml Flow Cytometry: ? 0.1 µg/106 cells. |
Reactivities | Mouse |
Conjugation | PE |