Secondary Antibodies

Rabbit IgG F(c) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rabbit
Conjugation Unconjugated

F(ab')2 Rabbit IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE

Rabbit IgG (H+L chain) sheep polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation TRITC

Rabbit IgM (B Cell Marker) mouse monoclonal antibody, clone NRBM, Purified

Applications Immunohistochemistry on Frozen Sections.
Flow Cytometry:
Use 10 µl of diluted antibody to label 1x10e6 cells in 100 µl.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgG mouse monoclonal antibody, clone 2A9, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG mouse monoclonal antibody, clone 2A9, Purified

Applications ELISA: 1/4,000-1/8,000.
Flow Cytometry.
FLISA.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Rabbit
Conjugation Unconjugated

Rabbit IgA goat polyclonal antibody, AP

Applications ELISA: 1/100-1/1000.
Reactivities Rabbit
Conjugation AP

Rabbit IgG (H+L chain) sheep polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immuno-peroxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer andspecificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) sheep polyclonal antibody, PE

Applications Flow Cytometry (Use 50 µl of 1/5-1/10 diluted antibody to label 10e6 cells in 100 µl).
Reactivities Rabbit
Conjugation PE

Rabbit IgG mouse monoclonal antibody, clone 2A9, PE

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Reactivities Rabbit
Conjugation PE

Rabbit IgG mouse monoclonal antibody, clone 2A9, FITC

Applications Flow Cytometry: < / = 3 µg/10e6 cells.
Reactivities Rabbit
Conjugation FITC

Rabbit IgG mouse monoclonal antibody, clone 2A9, AP

Applications ELISA: 1/2,000-1/4,000.
Reactivities Rabbit
Conjugation AP