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Secondary Antibodies
Monkey IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | Dot blot. Immunoblotting. ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. This antibody can be used: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histo- and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000. |
| Reactivities | Monkey |
| Conjugation | HRP |
Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
| Reactivities | Monkey |
| Conjugation | HRP |
FITC Conjugated Goat Anti-Mouse IgG
| Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
| Reactivities | Mouse IgG |
| Conjugation | FITC |
Rabbit Monoclonal Anti-Human IgM antibody, clone RM121
| Applications | ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL |
| Reactivities | Human IgM |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified
| Applications | Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. For use in ELISA a dilution of 1:3,000-1:12,000 is recommended. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
| Applications | Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE
| Applications | FLISA: ? 0.1 µg/ml Flow Cytometry: ? 0.1 µg/106 cells. |
| Reactivities | Mouse |
| Conjugation | PE |
Monkey IgA (Secretory component) goat polyclonal antibody, HRP
| Applications | Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working dilutions: Histochemical and cytochemical 1/150 - 1/500. ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000. |
| Reactivities | Chimpanzee, Monkey |
| Conjugation | HRP |
优化搜索结果
Reactivities
- Mouse (498)
- Human (432)
- Rat (196)
- Rabbit (150)
- Goat (80)
- Chicken (77)
- Monkey (69)
- Sheep (65)
- Bovine (45)
- Guinea Pig (45)
- Hamster (39)
- Canine (34)
- Feline (30)
- Porcine (28)
- Horse (21)
- Dog (18)
- Pig (14)
- Duck (12)
- Equine (9)
- Donkey (7)
- Golden Syrian Hamster (7)
- Turkey (6)
- Chimpanzee (4)
- Mouse IgG (3)
- Human IgA (2)
- Human IgG (2)
- Rabbit IgG (2)
- Reptiles (2)
- Goat IgG (1)
- Human Ig light chain (1)
- Human IgA1 (1)
- Human IgA2 (1)
- Human IgD (1)
- Human IgE (1)
- Human IgG1 (1)
- Human IgG3 (1)
- Human IgM (1)
- Human gamma heavy chain (1)
- Human kappa light chain (1)
- Human lambda light chain (1)
- Mammalian (1)
- Mouse Ig kappa light chain (1)
- Mouse IgG gamma (1)
- Mouse IgG1 (1)
- Mouse IgG2a kappa (1)
- Mouse IgG2b (1)
- Mouse IgM lambda light chain (1)
- Mouse IgM mu-chain (1)
- Rabbit IgG Fab (1)
- Rabbit IgG Fc (1)
Applications
Antibody Hosts
Conjugates
- Unconjugated (713)
- FITC (456)
- HRP (449)
- Biotin (334)
- TRITC (243)
- Texas Red (206)
- AP (151)
- Alkaline Phosphatase (87)
- PE (60)
- Phycoerythrin (21)
- ATTO 647N (15)
- ATTO 425 (14)
- ATTO 532 (14)
- ATTO 550 (14)
- ATTO 594 (14)
- ATTO 488 (13)
- ATTO 655 (13)
- Cy5 (6)
- APC (5)
- Cy3 (4)
- Agarose (3)
- BGAL (1)
- BIMA (1)
- PE-Cy7 (1)
