Secondary Antibodies

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/25,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemisstry: 1/1,000-1/5,000.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human
Conjugation Unconjugated

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 101, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Biotin

Applications ELISA: This antibody may be used in a Direct ELISA or as a Detection antibody in a Sandwich ELISA together with product code SM1508P as a Capture reagent.
Reactivities Canine, Feline, Human, Monkey
Conjugation Biotin

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000-1/100,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.

Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/40,000.
IHC: 1/1,000-1/5,000
Western Blot: 1/2,000-1/10,000.
Reactivities Human
Conjugation Unconjugated

Human IgA (alpha chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation Texas Red

Human IgA (alpha chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow cytometry: 1:2,000 - 1:10,000.
Immunflourescence microscopy: 1:500 - 1:2,500.
Reactivities Human
Conjugation FITC

Human IgM (Mu chain specific) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation Texas Red

Human IgM (Mu chain specific) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation TRITC

Human IgM (Mu chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation FITC

Human IgM (Mu chain specific) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (1:2,000 - 1:10,000), ELISA (1:20,000 - 1:100,000), Immunohistochemistry (1:1,000 - 1,5,000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:3,000 to 1:17,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgA (alpha chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin