Secondary Antibodies

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone 47.2.E9, Purified

Applications ELISA (1/5000-1/10000)
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes.
Positive control: Tonsil.
Cellular localization: Cytoplasmic.
Reactivities Human
Conjugation Unconjugated

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes.
Positive control: Tonsil.
Cellular localization: Cytoplasmic.
Reactivities Human
Conjugation Unconjugated

Human IgD mouse monoclonal antibody, clone IADB6, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone SB2H2, Purified

Applications ELISA.
Reactivities Human, Rabbit
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 3H10G5F2, Purified

Applications ELISA: 1/10000.
Reactivities Human
Conjugation Unconjugated

Goat anti-rabbit IgG Antiserum, pretitered, polyclonal

Reactivities Human, Mammalian
Conjugation Unconjugated

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, Purified

Applications ELISA: 1/500-1/1000.
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy,  Fluorescent based plate assays (FLISA) and  Fluorescent Western Blotting.
Also Suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. 
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy : 1/1,000-1/5,000.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500
Note:This antibody has been assayed against 1.0 ug of Human IgG F(c) in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Unconjugated