Secondary Antibodies

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Reactivities Hamster
Conjugation FITC

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Reactivities Guinea Pig
Conjugation Texas Red

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Guinea Pig
Conjugation TRITC

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Recommended dilutions:
Flow cytometry: 1:100 - 1:200
IF Mocroscopy: 1:100 - 1:200
Reactivities Human
Conjugation PE

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Reactivities Hamster
Conjugation TRITC

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Guinea Pig
Conjugation FITC

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Reactivities Guinea Pig
Conjugation HRP

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation TRITC

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:9,000 to 1:36,000 is suggested for this product.
Reactivities Rabbit
Conjugation Biotin

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rabbit
Conjugation Texas Red

Monkey IgM (Mu chain specific) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
This product has been assayed against 1.0 ug of Monkey IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/20,000 of the concentration is suggested for this product.
Reactivities Monkey
Conjugation Biotin

Monkey IgG (gamma chain specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:20,000 - 1:100,000 dilution.
Western Blot: 1:2000 - 1:10,000
Immunohistochemistry: 1:1000-1:5000
Reactivities Monkey
Conjugation Unconjugated

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/7,500-1/30,000.
Western Blot: 1/1000-1/5000.
Immunohistochemistry: 1/200-1/1000.
Note: This product has been assayed against 1.0 µg of Human IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/50,000 is suggested for this product.
Reactivities Human
Conjugation AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy (1:1,000-1:5,000) and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
ELISA : 1/10,000-1/50,000.
Reactivities Rabbit
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA : 1 :20,000-1 :100,000
Western blot : 1 :2,000-1 :10,000
Immunohistochemistry : 1 :1,000-1 :5,000.
Reactivities Sheep
Conjugation Unconjugated