Secondary Antibodies

Mouse IgM (Fc specific) rabbit polyclonal antibody, TRITC

Applications ELISA.
Immunocytochemistry.
(In)direct immunofluorescence

Immunohistochemistry on Frozen Sections.
Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/10- 1/80.
Reactivities Mouse
Conjugation TRITC

Mouse IgM (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
(In)direct immunofluorescence.
In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20- 1/80.
Reactivities Mouse
Conjugation FITC

Mouse IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used:
As unlabelled primary or secondary antibody reagent for indirect detection of IgM in Mouse cells, tissues and body fluids in Immunofluorescence and Immunoenzyme assay methods.
For the production of immunoconjugates with a selected marker.
To prepare immunoaffinity adsorbents by coupling to an artificial carrier.
In non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent.
In Western blotting.
When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before.
Recommneded Working Dilutions:
Histochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Precipitin titre: not less than 1/64 when tested against normal Mouse serum in agar-block immunodiffusion titration.

Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
Reactivities Mouse
Conjugation Unconjugated

Goat Anti-Mouse IgG (H+L) HRP conjugated secondary antibody

Applications EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user.
Reactivities Mouse
Conjugation HRP

Goat Anti-Mouse IgG (H+L) HRP conjugated secondary antibody

Applications EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/150,000.
Western Blot: 1/3,000-1/15,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2b Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgG3 Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
ELISA: 1:2,000 - 1:10,000
FLISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation TRITC

Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry.
Immunofluorescence:
≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) goat polyclonal antibody, TRITC

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation TRITC

Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry.
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC

Applications

FLISA: 1/100–1/400.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation TRITC