Secondary Antibodies

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Purified

Applications Flow Cytometry: Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA
FLISA
Immunohistochemistry (frozen sections).
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Purified

Applications ELISA (good binding on plastic) 
LO-MG-7 purified can be used at 0.6 µg/ml to obtain an OD of more than 1 on Mouse IgG2a en IgG2b.
Immunoassay: Useful as Secondary antibody.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Aff - Purified

Applications Anti-Mouse IgG1 secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/40,000-1/200,000.
Western Blot: 1/4,000-1/20,000. 
Immunohistochemistry: 1/2,000-1/10,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.

Ouchterlony Test:
Sample size: 15 µl.
Distance between wells: 5 mm.
Test Time: 5 days.
Temperature: 37°C.
Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
Results:
Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
Protein content: Biuret Assay 14 mg/ml.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM goat polyclonal antibody

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/2000 - 1/4000.
Western Blot.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a goat polyclonal antibody

Applications ELISA: 1/2000 - 1/4000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (adsorbed) goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. 
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified

Applications ELISA: 5 µg/ml as coating antibody.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on paraffin sections. 
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, Aff - Purified

Applications Immunoassay.
Immunohistochemistry on Frozen Sections. 
The specificity of monoclonal antibody anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at a high level can induce cross-reactions with other IgG subclasses.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Purified

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Purified

Applications Flow Cytometry: Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated