Secondary Antibodies

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), TRITC

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA, and Immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation TRITC

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:1,000 - 1:5,000
FLISA: 1:20,000 - 1:100,000
Conjugation TRITC

Mouse IgG3 Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
ELISA: 1:2,000 - 1:10,000
FLISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation TRITC

F(ab')2 Sheep IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Conjugation TRITC

F(ab')2 Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Conjugation TRITC

F(ab')2 Rabbit IgG Antibody Rhodamine Conjugated Pre-Adsorbed

Applications IF: 1:500-1:2,500
Conjugation TRITC

Human IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Reactivities Human
Conjugation TRITC

Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications ELISA.
Immunocytochemistry.
Immunofluorescence.
Immunohistochemistry Frozen Sections.

Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Porcine
Conjugation TRITC

Mouse IgG1 Antibody Rhodamine Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FLISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation TRITC

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for ELISA, Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA:  1/10,000 - 1/50,000.
IF Microscopy:  1/1000 - 1/5000.
Reactivities Goat
Conjugation TRITC

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, TRITC

Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.  
Recommended Dilutions: 
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500.
Reactivities Mouse
Conjugation TRITC

F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Applications IHC: 1:1000-1:5000
IF: 1:500-1:2,500
FC: 1:500-1:2,500
FLISA: 1:10,000-1:50,000
Conjugation TRITC

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Guinea Pig
Conjugation TRITC

Goat IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/1,000-1/5,000) and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Goat
Conjugation TRITC

Rabbit IgG (H+L chain) sheep polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation TRITC