Secondary Antibodies

Mouse IgM (Mu chain specific), adsorbed goat polyclonal antibody, HRP

Applications ELISA (1/1000).
Immunohistochemistry on Frozen and Paraffin Tissues (1/50).
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation HRP

Mouse IgG (adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/1000-1/10000.
Reactivities Mouse
Conjugation HRP

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation HRP

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, HRP, Purified

Applications

Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic),
immunohistochemistry on frozen sections and immunoaffinity chromatography.

Reactivities Mouse
Conjugation HRP

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, HRP, Purified

Applications Useful as second antibody in immunoassays, such as ELISA's (good binding on plastic) and immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation HRP

Mouse IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/10,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/10,000-1/50,000.
Western Blot : 1/1,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgA (+IgG +IgM) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation HRP

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 μg of Mouse IgG2b in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgA (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse lambda light chain goat polyclonal antibody, HRP

Applications ELISA (1/4,000-1/8,000).
Reactivities Mouse
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, HRP

Applications ELISA: 0.5 µg/ml as coating antibody.
Reactivities Mouse
Conjugation HRP