Secondary Antibodies

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Biotin, Aff - Purified

Applications Anti-Mouse IgG1 Biotin Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA: 1/5000-1/10000.
Enzyme-Linked-Immunosorbent-Assay.
Reactivities Mouse
Conjugation Biotin

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Biotin

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:550,000
Reactivities Mouse
Conjugation Biotin

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation Biotin

Biotin Conjugated Mouse IgG (H&L) Secondary Antibody Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:550,000
Reactivities Mouse
Conjugation Biotin

Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:3,000 - 1:30,000
IHC: 1:1,500 - 1:15,000
ELISA: 1:300,000
Reactivities Mouse
Conjugation Biotin

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions
ELISA: 1/20,000-1/100,000.
Immunohistochemistry: 1/1,000-1/5,000.
Western blot: 1/2,000-1/10,000.
Reactivities Mouse
Conjugation Biotin

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Biotin

Applications Flow Cytometry: < / = 1µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation Biotin

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Biotin, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation Biotin