Human IgG F(c) Antibody Pre-Adsorbed
Applications | WB: 1:1,000 - 1:5,000 IHC: 1:1,000 - 1:3,000 ELISA: 1:15,000 - 1:60,000 |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgG F(c) Antibody Pre-Adsorbed
Applications | WB: 1:1,000 - 1:5,000 IHC: 1:1,000 - 1:3,000 ELISA: 1:15,000 - 1:60,000 |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgA (alpha chain) rabbit polyclonal antibody, Aff - Purified
Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA: 1/5,000-1/20,000. Western blot: 1/500-1/2,000. IHC: 1/250-1:1,000 |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified
Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/2,000. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgA rabbit polyclonal antibody, Purified
Applications | Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100). Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Incubation Time: 30 minutes at room temperature. Positive Control: Tonsil. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgA rabbit polyclonal antibody, Purified
Applications | Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100). Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Incubation Time: 30 minutes at room temperature. Positive Control: Tonsil. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgG (Fc specific) rabbit polyclonal antibody, FITC
Applications | This Fluorescein Conjugated antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF MIscroscopy: 1/1000-1/5000. |
Reactivities | Human |
Conjugation | FITC |
Human IgE (Fc specific) goat polyclonal antibody, TRITC
Applications | Can be used for direct Immunofluorescence staining of cytoplasmic IgE of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/30. |
Reactivities | Human |
Conjugation | TRITC |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
Applications | Can be used as unlabelled secondary antibody for Indirect detection of IgE in Human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgD (Fc specific) goat polyclonal antibody, TRITC
Applications | Can be used in direct Immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: 1/10 - 1/30. |
Reactivities | Human |
Conjugation | TRITC |
Human IgD (Fc specific) goat polyclonal antibody, FITC
Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/40, depending on the method used. |
Reactivities | Human |
Conjugation | FITC |
Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
Reactivities | Human |
Conjugation | Unconjugated |
Human IgE goat polyclonal antibody, AP
Applications | ELISA: 1/1000-1/10000. Western Blot: 1/1000-1/5000. |
Reactivities | Human, Monkey |
Conjugation | AP |
Human IgE goat polyclonal antibody, FITC
Applications | Flow Cytometry. |
Reactivities | Human, Monkey |
Conjugation | FITC |
Human IgG (H&L) Antibody Fluorescein Conjugated
Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
Reactivities | Human |
Conjugation | FITC |
Human IgA (alpha chain) Antibody Fluorescein Conjugated
Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
Reactivities | Human |
Conjugation | FITC |