Secondary Antibodies

Rat IgE (Fc specific) goat polyclonal antibody, FITC

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To identify and measure IgE in Rat serum or other body fluids.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions: 1/20-1/80.
Reactivities Rat
Conjugation FITC

Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Applications IF: 1:100 - 1:250
FC: 1:100 - 1:250
Reactivities Rat
Conjugation Phycoerythrin

Rat IgG2ab (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used:
In immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of Rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user's choice has to be used.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays between: 1/2.000-1/10,000.
Reactivities Rat
Conjugation Biotin

Rat IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antibody can be used:
• As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids.
• In Immunofluorescence and immunoenzyme assay methods.
• For the production of immunoconjugates with a selected marker.
• To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier.
• As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat
Conjugation Unconjugated

Rat IgG2b rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgG1 goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgM goat polyclonal antibody, Purified

Applications ELISA 1ug/ml - 10ug/ml, Western Blotting, Flow Cytometry
Reactivities Rat
Conjugation Unconjugated

Rat IgA goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Immunofluorescence.
Reactivities Reptiles, Rat
Conjugation Unconjugated

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Purified

Applications ELISA.
Flow Cytometry:
5 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Reactivities Rat
Conjugation Unconjugated

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000. 
Western Blot. 
Immunofluorescence. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections.

Reactivities Rat
Conjugation Biotin

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Reactivities Rat
Conjugation HRP