Secondary Antibodies

Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications ELISA.
Immunocytochemistry.
Immunofluorescence.
Immunohistochemistry Frozen Sections.

Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Porcine
Conjugation TRITC

Porcine IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
Immunofluorescence.
Immunohistochemistry Frozen Sections.

Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Porcine
Conjugation FITC

Porcine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Porcine
Conjugation FITC

Porcine IgG F(c) specific goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Porcine
Conjugation FITC

Porcine IgG F(c) specific goat polyclonal antibody, AP

Applications ELISA: 1/5000-1/50000.
Reactivities Porcine
Conjugation AP

Porcine IgG F(c) specific goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000.
Reactivities Porcine
Conjugation Biotin

Porcine IgA goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgA goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/10000-1/100000
Reactivities Porcine
Conjugation Biotin

Porcine IgG (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Reactivities Porcine
Conjugation HRP

Porcine IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Porcine
Conjugation Unconjugated

Porcine IgG F(c) specific goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Porcine
Conjugation HRP