Secondary Antibodies

Human IgA (alpha chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/5,000-1/20,000.
Western blot: 1/500-1/2,000.
IHC: 1/250-1:1,000
Reactivities Human
Conjugation Unconjugated

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified

Applications Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA: 1/15,000-1/30,000
Western blot: 1/500-1/2,000
Immunohistochemistry: 1/500-1/2,000.
Reactivities Rabbit
Conjugation Unconjugated

Human IgA rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100).
Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining.
Incubation Time: 30 minutes at room temperature.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgA rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100).
Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining.
Incubation Time: 30 minutes at room temperature.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Guinea Pig IgG (H&L) Antibody Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
ELISA: 1:25,000 - 1:75,000
Conjugation Unconjugated

Monkey IgG (H&L) Antibody

Applications WB: 1:10,000 - 1:60,000
ELISA: 1:50,000 - 1:100,000
Conjugation Unconjugated

CNY 8,025.00

4周

VhH Antibody

Applications WB: 1:1,000-1:5,000
ELISA: 1:10,000-1:50,000
Conjugation Unconjugated

CNY 2,119.00

4周

VhH Antibody

Applications WB: 1:1,000-1:5,000
ELISA: 1:10,000-1:50,000
Conjugation Unconjugated

Human IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/2,000.
Reactivities Human
Conjugation Unconjugated

Porcine IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration.
Reactivities Porcine
Conjugation Unconjugated

Sheep IgG (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Conjugation Unconjugated

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry. 
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Aff - Purified

Applications

Indirect Immunostaining (In conjunction with mouse monoclonal antibodies).
Enzyme-Linked-Immunosorbent-Assay (ELISA)
Fluorescent-Linked-Immunosorbent-Assay (FLISA)
Immunoblotting. 
Flow Cytometry.

Reactivities Mouse
Conjugation Unconjugated