Secondary Antibodies

Rabbit Monoclonal Anti-Human Lambda Light Chain antibody, clone RM127

Applications ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL
Reactivities Human lambda light chain
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgM (mu-Chain) antibody, clone RM109

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Mouse IgM mu-chain
Conjugation Unconjugated

Goat Monoclonal Anti-Rabbit IgG Fc antibody, clone RMG02

Applications ELISA: 0.01ug/mL – 0.5ug/mL, ICC/IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Rabbit IgG Fc
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1-10 µg.
Reactivities Chicken
Conjugation Unconjugated

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000.
Immunofluorescence.
Reactivities Human
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Azide Free

Applications ELISA.
FLISA: 1 µg/ml.
Western blotting.
Dot- and Slot-Immunoblotting
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, HRP

Applications ELISA: 500 ng/ml.
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with SM1491P as the capture reagent.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (See Ref.1-5)
Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Human IgG mouse monoclonal antibody, clone 4D2D9G8, Purified

Applications Western Blot: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Reactivities Human
Conjugation Unconjugated

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation PE