Secondary Antibodies

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), HRP

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/25-1/100.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgD mouse monoclonal antibody, clone NI 158, HRP

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/500.
ELISA: from 1/1000 upwards.
Western blot: from 1/2000 upwards.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), HRP

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgE mouse monoclonal antibody, clone NI 307, HRP

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation HRP

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), HRP

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct Immunoperoxidase staining of cytoplasmic IgA2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), HRP

Applications To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/50 upwards
Western blot: from 1/100 upwards
Reactivities Human
Conjugation HRP

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), HRP

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/60 upwards.
Western blot: from 1/20 upwards.
Reactivities Human
Conjugation HRP

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Rat IgE mouse monoclonal antibody, clone MARE-1, HRP

Applications ELISA: 0.5 µg/ml.
Western Blot.
Reactivities Rat
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation HRP

Goat IgG (Fc specific) mouse monoclonal antibody, clone SB115d, HRP

Applications ELISA: 1/10,000-1/20,000.
Reactivities Goat
Conjugation HRP

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, HRP

Applications ELISA: 1/4,000-1/8,000 (See also Ref.1).
Reactivities Human
Conjugation HRP