Secondary Antibodies

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
  1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, FITC

Applications Flow Cytometry: 5 µg/ml; use 5 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, FITC

Applications FLISA: 1/200-1/600.
Indirect Immunofluorescent staining
of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
Reactivities Human
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, FITC

Applications Flow Cytometry: < / = 1.0 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation FITC

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, FITC, Purified

Applications

Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
The antibody can be used at 5 μg/ml.

Reactivities Mouse
Conjugation FITC

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Flow Cytometry: Use 50µl of 5µg/ml diluted antibody to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, FITC

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC