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Cy3 Conjugated Goat Anti-Rabbit IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Rabbit IgG |
| Conjugation | Cy3 |
Secondary Antibodies
Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Rabbit Monoclonal Anti-Human IgG1 Antibody, Clone RM117
| Applications | ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL |
| Reactivities | Human IgG1 |
| Conjugation | Unconjugated |
Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified
| Applications | ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen. Immunofluorescence. Immunohistochemistry on Frozen Sections. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Cy3 Conjugated Goat Anti-Mouse IgG
| Applications | IF/ICC: 1:50-100; IHC: 1:50-100 |
| Reactivities | Mouse IgG |
| Conjugation | Cy3 |
Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for ELISA, Immunofluorescence Microscopy. Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1000-1/5000. |
| Reactivities | Mouse |
| Conjugation | FITC |
Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified
| Applications | Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |
Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Rabbit Monoclonal Anti-Mouse IgG1 antibody, clone RM106
| Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB: 0.1ug/mL-0.5ug/mL |
| Reactivities | Mouse IgG1 |
| Conjugation | Unconjugated |
Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/3,000-1/25,000. Western blot: 1/1,000-1/4,000. Immunohistochemistry: 1/200-1/1,000. |
| Reactivities | Rabbit |
| Conjugation | AP |
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Reactivities
- Mouse (49)
- Human (38)
- Rabbit (23)
- Rat (13)
- Goat (8)
- Monkey (4)
- Canine (2)
- Chicken (2)
- Mouse IgG (2)
- Porcine (2)
- Bovine (1)
- Feline (1)
- Goat IgG (1)
- Hamster (1)
- Human Ig light chain (1)
- Human IgA1 (1)
- Human IgG (1)
- Human IgG1 (1)
- Mouse Ig kappa light chain (1)
- Mouse IgG gamma (1)
- Mouse IgG1 (1)
- Mouse IgM mu-chain (1)
- Pig (1)
- Rabbit IgG (1)
- Rabbit IgG Fab (1)
- Sheep (1)
