Secondary Antibodies

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/50-1/200.
ELISA: form 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-18132, Purified

Applications ELISA.
Recommended pairs for Sandwich Immunoassay:
Capture / Detection:
AM00919PU-N / AM00917PU-N.
AM00919PU-N / AM00918PU-N.
Reactivities Human
Conjugation Unconjugated

Human IgE (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80, depending on the method used.
Reactivities Human
Conjugation FITC

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards.
ELISA: form 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards
ELISA: form 1/25 upwards
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Biotin

Human IgE mouse monoclonal antibody, clone NI 307, FITC

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
Reactivities Human
Conjugation FITC

Human IgE (Fc specific) goat polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000.
Reactivities Human
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP

Applications ELISA: 1/4,000-1/10,000.
Western blotting.
Dot- and Slot-immunoblotting.
Immunohistochemistry on Frozen Sections: 1/10-1/20.
Reactivities Human
Conjugation HRP

Goat Polyclonal Anti-Human IgG Antibody

Applications WB 1:500-1:2,000
Reactivities Human
Conjugation Unconjugated

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/75,000-1/500,000.
Western Blot: 1/10,000-1/50,000.
Immunohistochemistry: 1/1,000-1/10,000.
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Applications Application(s): suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s):
ELISA: 1:25,000
Western Blot: 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,500
Reactivities Human
Conjugation Unconjugated

Human IgA (Secretory component) goat polyclonal antibody, FITC

Applications Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Human
Conjugation FITC

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Purified

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG4, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated