Secondary Antibodies

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Assay Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-5,000.
Reactivities Chicken
Conjugation FITC

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Biotin

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1/10.
Reactivities Chicken, Duck
Conjugation Biotin

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications ELISA.
Western Blot (1/5000).
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate.
Reactivities Chicken
Conjugation Biotin

Chicken IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Chicken
Conjugation FITC

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation Biotin

Chicken IgM goat polyclonal antibody, Purified

Applications ELISA: (1/100-1/1000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassay.
Western Blot  (non-recduced).
The typical starting working dilution is 1/10.
Immunoprecipitation. 
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck
Conjugation Unconjugated

Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Conjugation Unconjugated

PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose

Applications Immunoprecipitation.
Reactivities Chicken
Conjugation Agarose

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation PE