Secondary Antibodies

Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Texas Red

Applications Flow Cytometry: 1/2,000 - 1/10,000.
Immunofluorescence: 1/500 - 1/2,500.
Reactivities Sheep
Conjugation Texas Red

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:400,000 is suggested for ELISA.
Reactivities Sheep
Conjugation Biotin

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation AP

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:6,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation Biotin

Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation AP

Mouse lambda chain rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Guinea pig IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Reactivities Guinea Pig
Conjugation HRP

Rat IgA (alpha chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rat
Conjugation Texas Red

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000-1/12,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Mouse
Conjugation AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rabbit
Conjugation TRITC

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation Biotin

Rat IgM (Mu chain specific) goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Reactivities Rat
Conjugation PE

Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Reactivities Sheep
Conjugation PE

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Rabbit
Conjugation Texas Red

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry: the maximum amount of reagent required to stain 1x10e6 cells is approx. 1.0 µg of antibody conjugate.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Mouse
Conjugation PE