Secondary Antibodies

Human IgG rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature).
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Biotin

Applications ELISA: 1/5,000-1/10,000.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Biotin

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6029, Purified

Applications ELISA: Purified (UNLB) antibody ≤1 ug/mL.
Western blotting
Dot- and slot-immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Ffrozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone B3102E8, Purified

Applications ELISA (Recommended as a Detection Antibody).
Western blotting.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Purified

Applications Fluorescent-linked-immunosorbent assay (FLISA)
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry (frozen Sections).
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Purified

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA. 1 ug/mL
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Purified

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Low Endotoxin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC