Secondary Antibodies

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1-10 µg.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/64 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 08C, Purified

Applications ELISA.
Immunoblotting.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Purified

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Indirect Immunofluorescence staining. (Ref.1)
In ovo suppression of Chicken B lymphocytes.
In vitro activation of Chicken peripheral B lymphocytes.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (gamma chain specific) mouse monoclonal antibody, clone G-1, Purified

Applications ELISA.
Flow Cytometry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
Western blot (1/5000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Immunoprecipitation (1/250).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgM goat polyclonal antibody, Purified

Applications ELISA: (1/100-1/1000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassay.
Western Blot (non-recduced).
The typical starting working dilution is 1/10.
Immunoprecipitation.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, Purified

Applications ELISA (1/100-1/10000).
Immunodiffusion.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, Purified

Applications Flow Cytometry.
Immunoprecipitation. (Ref.1)
Reactivities Chicken
Conjugation Unconjugated

Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Conjugation Unconjugated

Chicken IgG / Chicken IgY (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reactivities Chicken
Conjugation Unconjugated