Secondary Antibodies

Human IgM (Fc specific) mouse monoclonal antibody, clone CH2, PE

Applications Flow cytometry: 0.5 µg/ml.
Reactivities Human
Conjugation PE

Human IgE mouse monoclonal antibody, clone BE5, PE

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, PE

Applications ELISA.
Immunofluorescence < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, PE

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgD mouse monoclonal antibody, clone IA6-2, PE

Applications Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities Human
Conjugation PE

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, PE

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 0.1 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, PE

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, PE

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, PE

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 0.1 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgG (adsorbed F(ab)2 Fragment) donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Reactivities Human
Conjugation PE

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in Flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Reactivities Human
Conjugation PE