Secondary Antibodies

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, FITC

Applications Flow cytometry: 5 µg/ml; use 10 µl of diluted antibody to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, FITC

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Rabbit IgG mouse monoclonal antibody, clone 2A9, FITC

Applications Flow Cytometry: < / = 3 µg/10e6 cells.
Reactivities Rabbit
Conjugation FITC

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Immunocytochemistry (1/200).
In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation FITC

Hamster IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/100 - 1/200.
Reactivities Hamster
Conjugation FITC

Rat IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/400 diluted antibody to label 1 x 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/500.
Reactivities Rat
Conjugation FITC

Cat IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FLISA: 1:10,000 - 1:50,000
Conjugation FITC