Secondary Antibodies

Mouse IgE (Fc specific) rabbit polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80, depending on the method used.
Reactivities Mouse
Conjugation FITC

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
 ≤ 1 µg/106 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation FITC

Mouse IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation FITC

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (See Ref.1-5)
Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Mouse IgM (Fc specific) goat polyclonal antibody, FITC

Applications In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Mouse
Conjugation FITC

Mouse IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/50-1/200 diluted antibody to label 1x106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, FITC, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Indirect Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Mouse IgG2b Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications

FLISA.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation FITC

Mouse IgG3 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC

Mouse IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/10.
Immunofluorescence: 1/10.
Reactivities Mouse
Conjugation FITC

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Mouse IgG2b goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10. 
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, FITC

Applications Flow Cytometry: 5 µg/ml; use 5 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC