Secondary Antibodies

Goat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/1,00,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Biotin

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Western Blot.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.

Reactivities Rat
Conjugation Biotin

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA: 1/5000-1/10000.
Enzyme-Linked-Immunosorbent-Assay.
Reactivities Mouse
Conjugation Biotin

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Flow Cytometry: 1 µg / 10e6 cells
Western Blotting
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation Biotin

Rabbit IgG mouse monoclonal antibody, clone 2A9, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin