Mouse IgG1 goat polyclonal antibody, AP
Applications | ELISA: 1/2000-1/4000. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. |
Reactivities | Mouse |
Conjugation | AP |
Mouse IgG1 goat polyclonal antibody, AP
Applications | ELISA: 1/2000-1/4000. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. |
Reactivities | Mouse |
Conjugation | AP |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
Reactivities | Mouse |
Conjugation | HRP |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin
Applications | ELISA: 1/5,000-1/20,000. |
Reactivities | Mouse |
Conjugation | Biotin |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE
Applications | FLISA: ? 0.1 µg/ml Flow Cytometry: ? 0.1 µg/106 cells. |
Reactivities | Mouse |
Conjugation | PE |
Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC
Applications | FLISA: 1/100–1/400. Flow Cytometry: ≤ 1 μg/106 cells. Immunofluorescence: ≤ 1 μg/106 cells. Immunohistochemistry. |
Reactivities | Mouse |
Conjugation | FITC |
Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free
Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. |
Reactivities | Mouse |
Conjugation | Unconjugated |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE
Applications | FLISA: ? 0.1 µg/ml Flow Cytometry: ? 0.1 µg/106 cells. |
Reactivities | Mouse |
Conjugation | PE |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free
Applications | Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
Reactivities | Mouse |
Conjugation | Unconjugated |
Mouse IgG1 goat polyclonal antibody, FITC
Applications | Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunocytochemistry: neat-1/10. |
Reactivities | Mouse |
Conjugation | FITC |
Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP
Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions Histochemical and cytochemical Use: 1/100- 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000. |
Reactivities | Mouse |
Conjugation | HRP |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
Reactivities | Mouse |
Conjugation | Biotin |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
Applications | ELISA: 1/5000-1/20000. |
Reactivities | Mouse |
Conjugation | Biotin |
Mouse IgM (Fc specific) goat polyclonal antibody, HRP
Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/400. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used. |
Reactivities | Mouse |
Conjugation | HRP |
Mouse IgE (Fc specific) goat polyclonal antibody, TRITC
Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/60, depending on the method used. |
Reactivities | Mouse |
Conjugation | TRITC |
Mouse IgG3 (subclass specific) goat polyclonal antibody, FITC
Applications | This antibody can be used to identify and measure IgG3, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: 1/20-1/80. |
Reactivities | Mouse |
Conjugation | FITC |