Secondary Antibodies

Human IgM mouse monoclonal antibody, clone NI 179, Purified

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, immunoblotting, immunoprecipitation, indirect immunoperoxidase and immunofluorescence
General Recommended Dilutions:
Histochemical use: 1/50-1/200
Dilutions may be prepared by adding PBS, pH 7.2.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1,000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Purified

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of
cytoplasmic IgG, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Ascites

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Ascites

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Azide Free

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards. 
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), Purified

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgD mouse monoclonal antibody, clone NI 158, Ascites

Applications To identify the presence of IgD in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgD and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/25 upwards.
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Purified

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmuno assay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250. 
ELISA: from 1/100 upwards. 
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin